Parameter interdependence and success of skeletal muscle modelling

In muscle and movement modelling it is almost invariably assumed that force actually exerted is determined by several independent factors. This review considers the fact that length force characteristics are not a relatively fixed property of muscle but should be considered the product of a substantial number of interacting factors. Level of activation and recruitment are influential factors in relation to aspects of muscle architecture. For the level of activation effects of its short term history (potentiation, fatigue in sustained contractions) have to be taken into account and are reviewed on the basis of recent experimental results as well as available literature. History is also an important determinant for the effect of length changes. This concept is introduced on the basis of recent experimental evidence as well as available literature. Regarding effects of muscle architecture, the concepts of primary and secondary distribution of fibre mean sarcomere length are introduced as well as effects of muscle geometry for mono- and bi-articular muscles on those distributions. Implications for motor control are discussed and the need for intramuscular coordination indicated

Muscle force is determined also by muscle relative position: isolated effects

Effects on force of changes of the position of extensor digitorum longus muscle (EDL) relative to surrounding tissues were investigated in rat. Connective tissue at the muscle bellies of tibialis anterior (TA), extensor hallucis longus (EHL) and EDL was left intact, to allow myofascial force transmission. The position of EDL muscle was altered, without changing EDL muscle–tendon complex length, and force exerted at proximal and distal tendons of EDL as well as summed force exerted at the distal tendons of TA and EHL muscles (TA+EHL) were measured. Proximal and distal EDL forces as well as distal TA+EHL force changed significantly on repositioning EDL muscle.\ud \ud These muscle position–force characteristics were assessed at two EDL lengths and two TA+EHL lengths. It was shown that changes of muscle force with length changes of a muscle is the result of the length changes per se, as well as of changes of relative position of parts of the muscle. It is concluded that in addition to length, muscle position relative to its surroundings co-determines isometric muscle force.\ud \ud Keywords: Intermuscular and extramuscular connective tissue; Myofascial force transmission; Rat m. extensor digitorum longus (EDL); Sarcomere length; Muscle relative positio

Communicating About Fascia: History, Pitfalls, and Recommendations

The modern reader and author need to be aware of possible ambiguities and misunderstandings stemming from different meanings of the word “fascia” because the general meaning of the term can be so vague as to imply little more than some form of connective tissue. “Fascia” encompasses both loose and dense, superficial and deep, and multiple- and single-layered connective tissues. To foster communication, we here suggest twelve specific terms to describe specified aspects of fascial tissue

Effects of knee joint angle on global and local strains within human triceps surae muscle: MRI analysis indicating in vivo myofascial force transmission between synergistic muscles

Purpose Mechanical interactions between muscles have been shown for in situ conditions. In vivo data for humans is unavailable. Global and local length changes of calf muscles were studied to test the hypothesis that local strains may occur also within muscle for which global strain equals zero. Methods For determination of globally induced strain in m. gastrocnemius in dissected human cadavers several knee joint angles were imposed, while keeping ankle joint angle constant and measuring its muscle-tendon complex length changes. In vivo local strains in both gastrocnemius and soleus muscles were calculated using MRI techniques in healthy human volunteers comparing images taken at static knee angles of 173° and 150°. Results Imposed global strains on gastrocnemius were much smaller than local strains. High distributions of strains were encountered, e.g. overall lengthened muscle contains locally lengthened, as well as shortened areas within it. Substantial strains were not limited to gastrocnemius, but were found also in synergistic soleus muscle, despite the latter muscle-tendon complex length remaining isometric (constant ankle angle: i.e. global strain = 0), as it does not cross the knee. Based on results of animal experiments this effect is ascribed to myofascial connections between these synergistic muscles. The most likely pathway is the neurovascular tract within the anterior crural compartment (i.e. the collagen reinforcements of blood vessels, lymphatics and nerves). However, direct intermuscular transmission of force may also occur via the perimysium shared between the two muscles. Conclusions Global strains imposed on muscle (joint movement) are not good estimators of in vivo local strains within it: differing in magnitude, as well as direction of length change. Substantial mechanical interaction occurs between calf muscles, which is mediated by myofascial force transmission between these synergistic muscles. This confirms conclusions of previous in situ studies in experimental animals and human patients, for in vivo conditions in healthy human subjects. © 2011 Springer-Verlag

Measuring wearing time of knee-ankle-foot orthoses in children with cerebral palsy: comparison of parent-report and objective measurement

Purpose State Orthotic wearing time may be an important confounder in efficacy studies of treatment in children with spastic cerebral palsy (SCP). Most studies measure parent-reported wearing time with questionnaires, but it is questionable whether this yields valid results. This study aims to compare parent-reported wearing time (WTparent) with objectively measured wearing time (WTobj) in children with SCP receiving orthotic treatment. Method Eight children with SCP participated in this observational study. For one year, they received knee-ankle-foot orthosis (KAFO) treatment. WTparent was measured using questionnaires. WTobj was measured using temperature sensor-data-loggers that were attached to the KAFOs. The 2.5th and 97.5th percentiles and median of differences between methods (per participant) were used to calculate limits of agreement and systematic differences. Results There was no systematic difference between WTparent and WTobj (0.1 hours per week), but high inter-individual variation of the difference was found, as reflected by large limits of agreement (lower limit/2.5th percentile: -1.7. hours/week; upper limit/97.5th percentile: 11.1 hours/week). Conclusions Parent-reported wearing time of a knee-ankle-foot orthosis differs largely from objectively measured wearing time using temperature sensors. Therefore, parent-reported wearing time of KAFOs should be interpreted with utmost care

Medidas de fração de vazio em escoamentos bifásicos por transmissão e difusão de nêutrons

Foram obtidas curvas de calibração que fornecem valores médios de fração de vazio (a), para misturas bifásicas de agua-vapor, para os regimes de escoamento a bolhas, bolsões ("slug"), anular e anular inverso. As medidas foram realizadas em modelos simulados de lucite-ar, para escoamento estacionário, pelas técnicas da transmissão e difusão de nêutrons térrmicos. As curvas de calibração obtidas foram utilizadas para medidas de fração de vazio em um circuito contendo mistura bifásica de água-ar, em escoamento concorrente ascendente, para os regimes a bolsões (pmax =1,06 bar) e anular (Pmax =1.33 bar), pelas mesmas técnicas anteriores. Em ambos os sistemas, utilizou-se uma seção de testes constituída de tubulação de alumínio (99,9%), com diâmetro interno de 25,25 mm e 2,00 mm de espessura de parede. O feixe de nêutrons foi obtido de uma fonte isotópica do tipo Am-8e, de 5 Cl, termalizados em uma blindagem cilíndrica de parafina de 500 mm de diâmetro (com H/D=l), recoberta com folhas de cadmio de 2 mm, tendo em seu interior um paralelepípedo de polietileno de alta densidade com dimensões de 240 x 240 x 144mm. Os nêutrons escapavam através de um colimador quadrangular paralelo de 53,00 x 25,25 mm, com 273 mm de comprimento, cavado em uma peça de parafina borada (32X de H3BO3). As medidas experimentais apresentaram boa concordância com os modelos teóricos da literatura especializada

Myofascial force transmission in dynamic muscle conditions: effects of dynamic shortening of a single head of multi-tendoned rat extensor digitorum longus muscle

This study investigated the effects of myofascial force transmission during dynamic shortening of head III of rat extensor digitorum longus muscle (EDL III). The anterior crural compartment was left intact. Force was measured simultaneously at the distal EDL III tendon, the proximal EDL tendon and the distal tendons of tibialis anterior and extensor hallucis longus muscles (TA+EHL). Two types of distal shortening of EDL III were studied: (1) sinusoidal shortening (2 mm) and (2) isokinetic shortening (8 mm). Sinusoidal shortening of EDL III caused a decrease in force exerted at the distal tendon of EDL III: from 0.58 (0.08) N to 0.26 (0.04) N. In contrast, hardly any changes in proximal EDL force and distal TA+EHL force were found. Maximal concentric force exerted at the distal tendon of EDL III was higher than maximal isometric force expected on the basis of the physiological cross-sectional area of EDL III muscle fibers (Maas et al. 2003). Therefore, a substantial fraction of this force must originate from sources other than muscle fibers of EDL III. Isokinetic shortening of EDL III caused high changes in EDL III force from 0.97 (0.15) N to zero. In contrast, changes in proximal EDL force were much smaller: from 2.44 (0.25) N to 1.99 (0.19) N. No effects on TA+EHL force could be shown. These results are explained in terms of force transmission between the muscle belly of EDL III and adjacent tissues. Thus, also in dynamic muscle conditions, muscle fiber force is transmitted via myofascial pathways. © Springer-Verlag 2005

Extramuscular myofascial force transmission alters substantially the acute effects of surgical aponeurotomy: assessment by finite element modeling

Effects of extramuscular myofascial force transmission on the acute effects of aponeurotomy were studied using finite element modeling and implications of such effects on surgery were discussed. Aponeurotomized EDL muscle of the rat was modeled in two conditions: (1) fully isolated (2) with intact extramuscular connections. The specific goal was to assess the alterations in muscle length-force characteristics in relation to sarcomere length distributions and to investigate how the mechanical mechanism of the intervention is affected if the muscle is not isolated. Major effects of extramuscular myofascial force transmission were shown on muscle length-force characteristics. In contrast to the identical proximal and distal forces of the aponeurotomized isolated muscle, substantial proximo-distal force differences were shown for aponeurotomized muscle with extramuscular connections (for all muscle lengths F dist > F prox after distal muscle lengthening). Proximal optimal length did not change whereas distal optimal length was lower (by 0.5 mm). The optimal forces of the aponeurotomized muscle with extramuscular connections exerted at both proximal and distal tendons were lower than that of isolated muscle (by 15 and 7%, respectively). The length of the gap separating the two cut ends of the intervened aponeurosis decreases substantially due to extramuscular myofascial force transmission. The amplitude of the difference in gap length was muscle length dependent (maximally 11.6% of the gap length of the extramuscularly connected muscle). Extramuscular myofascial force transmission has substantial effects on distributions of lengths of sarcomeres within the muscle fiber populations distal and proximal to the location of intervention: (a) Within the distal population, the substantial sarcomere shortening at the proximal ends of muscle fibers due to the intervention remained unaffected however, extramuscular myofascial force transmission caused a more pronounced serial distribution towards the distal ends of muscle fibers. (b) In contrast, extramuscular myofascial force transmission limits the serial distribution of sarcomere lengths shown for the aponeurotomized isolated muscle in the proximal population. Fiber stress distributions showed that extramuscular myofascial force transmission causes most sarcomeres within the aponeurotomized muscle to attain lengths favorable for higher force exertion. It is concluded that acute effects of aponeurotomy on muscular mechanics are affected greatly by extramuscular myofascial force transmission. Such effects have important implications for the outcome of surgery performed to improve impeded function since muscle in vivo is not isolated both anatomically and mechanically

Progressive surgical dissection for tendon transposition affects length-force characteristics of rat flexor carpi ulnaris muscle

Abstract Extramuscular connective tissue and muscular fascia have been suggested to form a myo-fascial pathway for transmission of forces over a joint that is additional to the generally accepted myo-tendinous pathway. The consequences of myo-fascial force transmission for the outcome of conventional muscle tendon transfer surgery has not been studied as yet. To test the hypothesis that surgical dissection of a muscle will affect its length-force characteristics, a study was undertaken in adult male Wistar rats. During progressive dissection of the flexor carpi ulnaris muscle, isometric length-force characteristics were measured using maximal electrical stimulation of the ulnar nerve. After fasciotomy, muscle active force decreased by approximately 20%. Further dissection resulted in additional decline of muscle active force by another 40% at maximal dissection. The muscle length at which the muscle produced maximum active force increased by approximately 0.7 mm (i.e. 14% of the measured length range) after dissection. It is concluded that, in rats, the fascia surrounding the flexor carpi ulnaris muscle is a major determinant of muscle length-force characteristics.